عنوان

High Performance Liquid Chromatography

3642670644

3642670660

9783642670640

9783642670664

b572481

High Performance Liquid Chromatography

[Book]

by Heinz Engelhardt.

Berlin, Heidelberg

Springer Berlin Heidelberg

1979

Anleitungen für die chemische Laboratoriumspraxis.

I. Chromatographic Processes --; References Chapter I --; II. Fundamentals of Chromatography --; A. Retention --; B. Linear Flow Rate, Porosity, Permeability --; C. Band Broadening --; D. Resolution --; E. Dependence of Band Broadening on Flow Rate --; F. Band Broadening and Particle Size --; G. Extra-Column Band Broadening --; H. Optimum Analysis Conditions and Analysis Time --; I. Selection of a Suitable Column --; References Chapter II --; III. Equipment for HPLC --; A. Solvent Reservoir --; Degassing of the Eluent --; B. Pumps --; C. Damping of the Pulsations --; D. Sample Introduction --; E. The Column --; F. Thermostating --; G. Measurement of the Flow Rate --; H. Fraction Collectors --; I. Recorders --; J. Instrumentation for Gradient Elution --; K. Safety Measures --; References Chapter III --; IV. Detectors --; A. UV Detectors --; B. Differential Refractometer --; C. Microadsorption Detector --; D. Transport Detector (Flame Ionization Detector) --; E. Fluorescence Detector --; F. Other Detectors --; G. Comparison of the Important Detectors --; H. Reaction Detectors --; References Chapter IV --; V. Stationary Phases --; A. Packing Materials for Adsorption and Partition Chromatography --; B. Chemically Modified Supports --; C. Ion Exchangers --; D. Stationary Phases for Exclusion Chromatography --; References Chapter V --; VI. Adsorption Chromatography --; I. Polar Stationary Phases --; II. Nonpolar Stationary Phases --; III. The General Elution Problem --; IV. Applications of Adsorption Chromatography --; References Chapter VI --; VII. Partition Chromatography --; A. Introduction --; B. Supports and Liquid Phases --; C. Properties of the Column --; D. Applications --; E. Ion-pair Chromatography --; References Chapter VII --; VIII. Ion-Exchange Chromatography --; A. Principle --; B. Ion Exchange Materials --; C. Characterization of the Ion Exchangers --; D. Optimizing a Separation --; E. Applications --; References Chapter VIII --; IX. Exclusion Chromatography. Gel Permeation Chromatography --; A. Introduction --; B. Basics of Exclusion Chromatography --; C. Stationary Phases for Exclusion Chromatography --; D. Applications of Exclusion Chromatography --; References Chapter IX --; X. Selection of the Separation System --; References Chapter X --; XI. Special Techniques --; A. Preparative Chromatography --; B. Qualitative Analysis --; C. Quantitative Analysis --; D. Trace Analysis --; References Chapter XI --; XII. Purification of Solvents --; References Chapter XII.

Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta­ cular development in analytical chemistry and has not yet abated be­ cause its vast potential for application remains to be fully exploit­ ed. Significant factors contributing to this continued rise are the simplicity and low cost of the required basic equipment and the rela­ tive ease of acquiring and interpreting the data. Unfortunately, in LC, as so often in the field of analytical chemistry, the available commercial instruments are frequently far more complicated - and consequently far more expensive - than is nec­ essary for routine application. Therein also lies the risk of propa­ gating a "black box" philosophy that would be particularly detrimen­ tal to chromatography. Moreover, it appears to have been forgotten, as was done previously with gas chromatography, that inadequate sep­ aration by a column can be remedied only with great difficulty, if at all, by electronic means. Also, whether the capillary columns recent­ ly advocated with great enthusiasm for LC will fulfill the expecta­ tions of their proponents is highly questionable unless someone comes up with some new and revolutionary ideas.

Analytical biochemistry.

Biochemistry.

Chemistry.

by Heinz Engelhardt.

Heinz Engelhardt

[Book]

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