عنوان

Next generation sequencing in forensic science

(Number (ISBN

9781003196464

(Number (ISBN

9780367478933

(Number (ISBN

9781032072043

Number

E3508

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Next generation sequencing in forensic science

Other Title Information

a primer

First Statement of Responsibility

Kelly M. Elkins and Cynthia B. Zeller.

Place of Publication, Distribution, etc.

Abingdon, Oxon

Name of Publisher, Distributor, etc.

CRC Press

Date of Publication, Distribution, etc.

2022.

Specific Material Designation and Extent of Item

xxx, 162 p.

Text of Note

Includes bibliographical references and index.

Text of Note

History of DNA-based human identification in forensic science -- History of sequencing for human DNA typing -- Sample preparation, standards and library preparation for next generation sequencing -- Performing next generation sequencing -- Next generation sequencing data analysis and interpretation -- Next generation sequencing troubleshooting -- Mitochondrial DNA typing using next generation sequencing -- Microbial applications of next generation sequencing for forensic investigations -- Body fluid analysis using next generation sequencing -- Conclusions and future outlook of next generation sequencing in forensic science.

0

Text of Note

"Next Generation Sequencing in Forensic Science: A Primer addresses Next Generation Sequencing (NGS) specific to its application to forensic science. The book opens with a history of human identity approaches including VNTR, RFLP, STR, and SNP DNA typing. It next discusses the history of sequencing for human DNA typing including: Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. Readers are expected to have as basic understanding of molecular and cellular biology and DNA typing. Chapters present an overview of the forensically-focused AmpliSeq, ForenSeq, Precision ID, PowerSeq and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs run on the MiSeq FGx and Ion Torrent System. Using these, that authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef including creating a sample list, wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained including how to analyze and interpret NGS data and output graphs and charts. The book concludes with the mitochondrial chromosome and mitochondrial DNA (mtDNA) sequencing and SNPs analysis including the issue of heteroplasmy. The final chapters review applications of microbial DNA for forensics, NGS in body fluid analysis, and challenges and considerations for future applications"--

Entry Element

DNA fingerprinting.

Entry Element

Forensic genetics.

Entry Element

Nucleotide sequence.

Entry Element

Bioinformatics.

Edition

Class number

Entry Element

Elkins, Kelly M.,

Entry Element

Zeller, Cynthia B.,

Country

ایران

Agency

University of Tehran. Library of College of Science

Date of Transaction

20220228141902.0

Cataloguing Rules (Descriptive Conventions))

rda

Date and Hour of Consultation and Access

UT_SCI_BL_DB_1003743_0001.pdf

e

BL

278840

a

Y